Reduction of malachite green to leucomalachite green by intestinal bacteria.
نویسندگان
چکیده
Intestinal microfloras from human, rat, mouse, and monkey fecal samples and 14 pure cultures of anaerobic bacteria representative of those found in the human gastrointestinal tract metabolized the triphenylmethane dye malachite green to leucomalachite green. The reduction of malachite green to the leuco derivative suggests that intestinal microflora could play an important role in the metabolic activation of the triphenylmethane dye to a potential carcinogen.
منابع مشابه
Toxicology and carcinogenesis studies of malachite green chloride and leucomalachite green. (CAS NOS. 569-64-2 and 129-73-7) in F344/N rats and B6C3F1 mice (feed studies).
BACKGROUND Malachite green chloride is a dye used to prevent fungus infections in commercial fisheries. Leucomalachite green is formed from malachite green and remains in the tissues of exposed fish. We studied the effects of malachite green on female rats and female mice, and the effects of leucomalachite green on male and female rats and female mice, to identify potential toxic or cancer-rela...
متن کاملEffects of Malachite Green and Its Major Metabolite, Leucomalachite Green, in Micromass Cultures of Rat Embryonic Cells
Embryonic mesenchyme limb (LB) and midbrain (CNS) cells cultured at high density were exposed to different concentrations of the fungicide malachite green (MG) and its major metabolite leucomalachite green (LMG). The concentration response curves were analysed, and two endpoints were calculated: the concentrations (μg/mL) that produced 50% inhibition of viability/proliferation (IC50-P) and diff...
متن کاملCollection and pre-selection of available data to be used for the risk assess-ment of malachite green residues by JECFA - Updated BfR Expert Opinion No. 007/2008 of 7 January 2008
Residues from the use of malachite green, a compound illegally applied as veterinary drug in aquacultures intended for human consumption, are frequently reported to occur in fish and fish products. Legally, zero tolerance applies to all residues of malachite green including its main metabolite leucomalachite green which is the dominating residue in tissues of exposed fish. In the European Union...
متن کاملApplication of graphene-based solid-phase extraction for ultra-fast determination of malachite green and its metabolite in fish tissues.
An ultra-fast analytical protocol using graphene-based solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry for the rapid determination of malachite green and its metabolite, leucomalachite green in fish tissues has been developed. In the present work, graphene was synthesized and evaluated as novel solid-phase extraction sorbents for the analytes...
متن کاملEnhanced Sensitive Immunoassay: Noncompetitive Phage Anti-Immune Complex Assay for the Determination of Malachite Green and Leucomalachite Green
To develop a more sensitive immunoassay for malachite green (MG) and leucomalachite green (LMG), we identified the immunocomplex binding phage-borne peptides for use in the noncompetitive phage anti-immunocomplex assay (PHAIA). An anti-LMG monoclonal antibody (mAb) was used to select immunocomplex binding peptides from a circular random eight-amino-acid phage-displayed library. After three roun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 63 10 شماره
صفحات -
تاریخ انتشار 1997